How to Reconstitute Peptides

What You Need

Before you start, gather everything. You don’t want to stop mid-process.

Essential supplies:

Item	Purpose	Notes
Peptide vial	The compound you’re reconstituting	Lyophilized (freeze-dried) powder. Keep refrigerated.
Bacteriostatic water (BAC water)	Solvent for mixing	Contains 0.9% benzyl alcohol preservative. NOT sterile water.
Mixing syringe	Transfer BAC water to peptide vial	3 mL syringe, 20-gauge needle, 1-inch length
Insulin syringes	Daily injections	U-100, 29–31 gauge, 0.5-inch needle. One per injection.
Alcohol prep pads	Sterilize vial tops and injection sites	Box of 100 lasts months
Sharps container	Safe syringe disposal	Any puncture-resistant container works

Where to buy: Pre-made kits from bacteriostaticwater.com or Amazon bundle everything except the peptide. Total cost for supplies: approximately $15–25.

Reconstitution Math

This is the part that trips people up. It’s just division.

Formula:

1. Concentration = Vial size (mcg) ÷ BAC water added (mL)
2. Volume per dose = Desired dose (mcg) ÷ Concentration (mcg/mL)
3. Units to draw = Volume (mL) × 100

A U-100 insulin syringe has 100 units per 1 mL. So 10 units = 0.10 mL, 20 units = 0.20 mL.

Common Reconstitution Charts

BPC-157 (5 mg vial + 2 mL BAC water)

Dose	Volume	Units
250 mcg	0.10 mL	10 units
300 mcg	0.12 mL	12 units
500 mcg	0.20 mL	20 units

Concentration: 2,500 mcg/mL. Vial lasts 20 days at 250 mcg/day.

TB-500 (5 mg vial + 1 mL BAC water)

Dose	Volume	Units
750 mcg	0.15 mL	15 units
2,000 mcg (2 mg)	0.40 mL	40 units
2,500 mcg (2.5 mg)	0.50 mL	50 units

Concentration: 5,000 mcg/mL. Vial lasts 2 doses at 2.5 mg.

GHK-Cu (50 mg vial + 5 mL BAC water)

Dose	Volume	Units
1,000 mcg (1 mg)	0.10 mL	10 units
2,000 mcg (2 mg)	0.20 mL	20 units

Concentration: 10,000 mcg/mL. Vial lasts 25 doses at 2 mg/day.

Need exact numbers for your vial? Use the Reconstitution Calculator.

How to Reconstitute (Step by Step)

1. Prepare Your Workspace

• Wash hands thoroughly
• Work on a clean, flat surface
• Remove the peptide vial from the fridge. Let it sit at room temperature for 10–15 minutes (reduces condensation)

2. Open and Sterilize

• Pop the plastic flip cap off the peptide vial to expose the rubber stopper
• Wipe the rubber stopper with an alcohol prep pad. Let it air dry.
• Wipe the BAC water bottle stopper the same way.

3. Draw the BAC Water

• Using the mixing syringe (3 mL, 20-gauge), draw the desired amount of BAC water
• Pull back slowly to minimize air bubbles
• For a 5 mg vial, draw 2 mL (2 cc)

4. Add Water to the Peptide Vial

• Insert the mixing syringe needle through the peptide vial’s rubber stopper
• Aim the stream at the glass wall — not directly onto the powder
• Inject slowly, letting the water run down the side of the vial
• This prevents foaming and peptide degradation

5. Dissolve

• Remove the syringe gently
• Swirl the vial gently between your fingers until the powder dissolves
• Never shake. Shaking can denature the peptide.
• Should dissolve in 30–60 seconds into a clear, colorless solution
• If particles remain, let the vial sit for 10–20 minutes and swirl again

6. Label and Store

• Label the vial with: compound name, concentration, and date
• Example: “BPC-157, 2,500 mcg/mL, Feb 18 2026”
• Refrigerate immediately at 2–8°C (36–46°F)

You only do this once per vial. Every injection after this is just drawing from the reconstituted solution.

How to Inject (Subcutaneous)

1. Prepare

• Remove the reconstituted vial from the fridge. Let it warm 5–10 minutes. Cold injections sting.
• Wash hands.
• Wipe the vial stopper with an alcohol prep pad.

2. Draw Your Dose

• Using a fresh insulin syringe, insert the needle through the rubber stopper
• Turn the vial upside down
• Pull back the plunger to your dose (e.g., 10 units for 250 mcg from a 2,500 mcg/mL solution)
• Tap out any air bubbles. Push the plunger slightly to expel them.
• Remove the needle from the vial.

3. Choose Your Injection Site

Best sites for subcutaneous injection:

Site	Notes
Lower abdomen	2+ inches from navel. Most popular. Most tissue to work with.
Front of thigh	Midway between hip and knee. Easy to reach.
Upper arm (tricep area)	Harder to reach solo. Good rotation option.

For BPC-157 specifically: Injecting near the injury site may provide faster local effects, though systemic distribution occurs regardless of injection location.

4. Inject

1. Clean the injection site with an alcohol prep pad. Let it air dry.
2. Pinch a fold of skin between your thumb and forefinger.
3. Insert the needle at a 45-degree angle into the pinched skin fold.
4. Push the plunger slowly and steadily. Almost no resistance.
5. Remove the needle. Release the skin fold.
6. Don’t rub. Light pressure with a clean pad if there’s any minor bleeding.
7. Dispose of the syringe in a sharps container. Never recap and reuse.

5. Rotate Sites

Alternate between at least 4 sites systematically:

1. Left abdomen
2. Right abdomen
3. Left thigh
4. Right thigh

Using the same spot every day causes lipohypertrophy (hard lumps under the skin). Space injections at least 1–2 inches apart within the same general area.

Storage Rules

State	Storage	Duration
Unreconstituted powder	Refrigerator (preferred) or freezer	Months to years
Reconstituted solution	Refrigerator, 2–8°C	28–30 days max
Pre-loaded syringe	Refrigerator, needle-up	3–5 days max

Never freeze reconstituted peptide. Freezing destroys the molecular structure.

Discard if: Solution is cloudy, has visible particles, changes color, or has been at room temperature for extended periods.

Protect from light: Store vials in the original box or wrap in foil. UV light degrades most peptides.

BAC Water vs. Sterile Water

	BAC Water	Sterile Water
Preservative	0.9% benzyl alcohol	None
Multi-use	Yes — safe for 28 days	No — use within 24 hours
Cost	~$5–8 per 30 mL	~$3–5 per 30 mL
Use case	Daily injection protocols	Single-use reconstitution

Always use BAC water for peptide protocols that span multiple days. Sterile water is only appropriate if you’ll use the entire vial in a single session.

Troubleshooting

Powder won’t dissolve: Let it sit for 10–20 minutes, then gently swirl again. Some peptides take longer. If it still won’t dissolve after 30 minutes, the peptide may be degraded.

Solution is cloudy: Discard. Cloudy solution indicates bacterial contamination or peptide aggregation. Do not inject.

Air bubbles in syringe: Tap the syringe barrel with your finger to move bubbles to the top, then push the plunger slightly to expel them. Small bubbles in a subcutaneous injection are harmless but reduce dose accuracy.

Injection site bleeds: Normal. A small drop of blood at the needle entry point is common. Apply light pressure for 10 seconds.

Injection site is red/itchy: Mild redness and itching are the most common side effects across all peptides. Usually resolves within 1–2 hours. If it persists beyond 24 hours or worsens, stop and reassess.

Stinging during injection: The peptide was too cold. Let it warm to room temperature (5–10 minutes) before drawing. Some compounds sting regardless — this is normal and harmless.